What are the biosynthetic and catabolic pathways in biochemistry?
The biosynthetic pathways include
- step 1 → synthesis of glucose from non-carbohydrate sources like amino acids, lactate, and glycerol through gluconeogenesis
- step 2 → synthesis of fatty acids from acetyl-CoA through lipogenesis
- step 3 → synthesis of proteins from amino acids through protein synthesis The catabolic pathways include
- step 1 → breakdown of glucose to pyruvate through glycolysis
- step 2 → breakdown of fatty acids to acetyl-CoA through lipolysis
- step 3 → breakdown of proteins to amino acids through proteolysis
What is the importance of lipids in the body?
Lipids are important for
- energy storage
- cell membrane structure
- hormone production
- absorption of vitamins
What are the different properties of lipids?
Lipids have the following properties
- insolubility in water
- solubility in organic solvents
- high energy density
- hydrophobic nature
What are the different types of carbohydrates?
Carbohydrates can be classified into
- monosaccharides like glucose and fructose
- disaccharides like sucrose and lactose
- polysaccharides like starch and glycogen
What are the major functions of carbohydrates?
Carbohydrates have the following functions
- energy source
- cell wall structure
- storage form of energy
- component of glycoproteins and glycolipids
What are the food sources of carbohydrates?
Carbohydrates are found in
- grains like rice and wheat
- fruits like apples and bananas
- vegetables like potatoes and carrots
- legumes like beans and lentils
What is the process of glycolysis?
Glycolysis is the breakdown of glucose to pyruvate
- step 1 → glucose is converted to glucose-6-phosphate
- step 2 → glucose-6-phosphate is converted to fructose-6-phosphate
- step 3 → fructose-6-phosphate is converted to fructose-1,6-bisphosphate
- step 4 → fructose-1,6-bisphosphate is converted to pyruvate
What is the process of gluconeogenesis?
Gluconeogenesis is the synthesis of glucose from non-carbohydrate sources
- step 1 → pyruvate is converted to oxaloacetate
- step 2 → oxaloacetate is converted to phosphoenolpyruvate
- step 3 → phosphoenolpyruvate is converted to glucose
What is the process of ATP production through oxidative phosphorylation?
ATP production through oxidative phosphorylation involves
- step 1 → electrons are passed through a series of electron transport chains
- step 2 → protons are pumped across the mitochondrial membrane
- step 3 → protons flow back across the membrane through ATP synthase
- step 4 → ATP is produced from ADP and Pi
What are the special features of protein metabolism?
Protein metabolism has the following special features
- continuous synthesis and breakdown of proteins
- regulation of protein synthesis by hormones and growth factors
- importance of protein turnover for maintenance of tissue health
What are the functions of intact amino acids?
Intact amino acids have the following functions
- building blocks of proteins
- precursors for synthesis of other biomolecules
- regulators of metabolic pathways
What is the oxidation of amino acids?
Oxidation of amino acids involves
- step 1 → removal of amino group through transamination or deamination
- step 2 → conversion of amino acid to keto acid
- step 3 → oxidation of keto acid to acetyl-CoA or other intermediates
What is the synthesis of proteins?
Protein synthesis involves
- step 1 → transcription of DNA to mRNA
- step 2 → translation of mRNA to protein
- step 3 → post-translational modification of protein
What is the function of nitrogenous part?
The nitrogenous part of amino acids has the following functions
- formation of urea for excretion of excess nitrogen
- synthesis of other nitrogen-containing biomolecules
What is the exogenous and endogenous protein metabolism?
Exogenous protein metabolism refers to the digestion and absorption of dietary proteins
- step 1 → digestion of proteins to amino acids
- step 2 → absorption of amino acids into bloodstream Endogenous protein metabolism refers to the synthesis and breakdown of proteins within the body
- step 1 → synthesis of proteins from amino acids
- step 2 → breakdown of proteins to amino acids
What is the concept of enzyme, chemical reactions, catalyst and substrates?
Enzymes are biological catalysts that speed up chemical reactions
- step 1 → binding of substrate to enzyme
- step 2 → conversion of substrate to product
- step 3 → release of product from enzyme
What are the major functions of enzymes?
Enzymes have the following functions
- catalysis of chemical reactions
- regulation of metabolic pathways
- maintenance of tissue health
What is the importance of enzymes in the body?
Enzymes are important for
- maintenance of homeostasis
- regulation of metabolic pathways
- synthesis and breakdown of biomolecules
What is the definition of vitamin?
Vitamins are organic compounds that are essential for maintenance of health
- they are not synthesized by the body in sufficient amounts
- they must be obtained from the diet
How are vitamins classified?
Vitamins are classified into
- fat-soluble vitamins like vitamins A, D, E, and K
- water-soluble vitamins like vitamins B and C
What are common vitamin deficiencies?
Common vitamin deficiencies include
- vitamin A deficiency leading to night blindness
- vitamin D deficiency leading to rickets
- vitamin B12 deficiency leading to anemia
What is the use of different instruments in biochemistry experiments?
Different instruments are used for
- measurement of pH and temperature
- separation and identification of biomolecules
- determination of concentration of biomolecules
How to perform the qualitative analysis of carbohydrate?
Qualitative analysis of carbohydrate involves
- step 1 → preparation of sample
- step 2 → addition of reagents
- step 3 → observation of color change or precipitation
How to interpret the results of qualitative analysis of carbohydrate?
Results are interpreted based on
- color change or precipitation
- presence or absence of carbohydrate
How to perform the qualitative analysis of protein?
Qualitative analysis of protein involves
- step 1 → preparation of sample
- step 2 → addition of reagents
- step 3 → observation of color change or precipitation
How to interpret the results of qualitative analysis of protein?
Results are interpreted based on
- color change or precipitation
- presence or absence of protein
How to perform the qualitative analysis of lipid?
Qualitative analysis of lipid involves
- step 1 → preparation of sample
- step 2 → addition of reagents
- step 3 → observation of color change or precipitation
How to interpret the results of qualitative analysis of lipid?
Results are interpreted based on
- color change or precipitation
- presence or absence of lipid
How to perform the quantitative estimation of glucose?
Quantitative estimation of glucose involves
- step 1 → preparation of sample
- step 2 → addition of reagents
- step 3 → measurement of absorbance or concentration
How to interpret the results of quantitative estimation of glucose?
Results are interpreted based on
- concentration of glucose
- comparison with standard values
How to perform the quantitative estimation of total proteins?
Quantitative estimation of total proteins involves
- step 1 → preparation of sample
- step 2 → addition of reagents
- step 3 → measurement of absorbance or concentration
How to interpret the results of quantitative estimation of total proteins?
Results are interpreted based on
- concentration of total proteins
- comparison with standard values
How to observe the quantitative estimation of uric acid?
Quantitative estimation of uric acid involves
- step 1 → preparation of sample
- step 2 → addition of reagents
- step 3 → measurement of absorbance or concentration
How to interpret the results of quantitative estimation of uric acid?
Results are interpreted based on
- concentration of uric acid
- comparison with standard values
How to observe the laboratory testing for lipid profile?
Laboratory testing for lipid profile involves
- step 1 → preparation of sample
- step 2 → measurement of lipid components
- step 3 → interpretation of results
How to interpret the results of lipid profile testing done in a laboratory?
Results are interpreted based on
- concentration of lipid components
- comparison with standard values
- presence or absence of lipid disorders