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Physiology - First Year BHMS

Contents

Physiology - First Year BHMS

Contents

CoursesBHMSPhysiology - First Year BHMSBIOCHEMISTRY

BIOCHEMISTRY

Content

What are the biosynthetic and catabolic pathways in biochemistry?

The biosynthetic pathways include

  • step 1 → synthesis of glucose from non-carbohydrate sources like amino acids, lactate, and glycerol through gluconeogenesis
  • step 2 → synthesis of fatty acids from acetyl-CoA through lipogenesis
  • step 3 → synthesis of proteins from amino acids through protein synthesis The catabolic pathways include
  • step 1 → breakdown of glucose to pyruvate through glycolysis
  • step 2 → breakdown of fatty acids to acetyl-CoA through lipolysis
  • step 3 → breakdown of proteins to amino acids through proteolysis

What is the importance of lipids in the body?

Lipids are important for

  • energy storage
  • cell membrane structure
  • hormone production
  • absorption of vitamins

What are the different properties of lipids?

Lipids have the following properties

  • insolubility in water
  • solubility in organic solvents
  • high energy density
  • hydrophobic nature

What are the different types of carbohydrates?

Carbohydrates can be classified into

  • monosaccharides like glucose and fructose
  • disaccharides like sucrose and lactose
  • polysaccharides like starch and glycogen

What are the major functions of carbohydrates?

Carbohydrates have the following functions

  • energy source
  • cell wall structure
  • storage form of energy
  • component of glycoproteins and glycolipids

What are the food sources of carbohydrates?

Carbohydrates are found in

  • grains like rice and wheat
  • fruits like apples and bananas
  • vegetables like potatoes and carrots
  • legumes like beans and lentils

What is the process of glycolysis?

Glycolysis is the breakdown of glucose to pyruvate

  • step 1 → glucose is converted to glucose-6-phosphate
  • step 2 → glucose-6-phosphate is converted to fructose-6-phosphate
  • step 3 → fructose-6-phosphate is converted to fructose-1,6-bisphosphate
  • step 4 → fructose-1,6-bisphosphate is converted to pyruvate

What is the process of gluconeogenesis?

Gluconeogenesis is the synthesis of glucose from non-carbohydrate sources

  • step 1 → pyruvate is converted to oxaloacetate
  • step 2 → oxaloacetate is converted to phosphoenolpyruvate
  • step 3 → phosphoenolpyruvate is converted to glucose

What is the process of ATP production through oxidative phosphorylation?

ATP production through oxidative phosphorylation involves

  • step 1 → electrons are passed through a series of electron transport chains
  • step 2 → protons are pumped across the mitochondrial membrane
  • step 3 → protons flow back across the membrane through ATP synthase
  • step 4 → ATP is produced from ADP and Pi

What are the special features of protein metabolism?

Protein metabolism has the following special features

  • continuous synthesis and breakdown of proteins
  • regulation of protein synthesis by hormones and growth factors
  • importance of protein turnover for maintenance of tissue health

What are the functions of intact amino acids?

Intact amino acids have the following functions

  • building blocks of proteins
  • precursors for synthesis of other biomolecules
  • regulators of metabolic pathways

What is the oxidation of amino acids?

Oxidation of amino acids involves

  • step 1 → removal of amino group through transamination or deamination
  • step 2 → conversion of amino acid to keto acid
  • step 3 → oxidation of keto acid to acetyl-CoA or other intermediates

What is the synthesis of proteins?

Protein synthesis involves

  • step 1 → transcription of DNA to mRNA
  • step 2 → translation of mRNA to protein
  • step 3 → post-translational modification of protein

What is the function of nitrogenous part?

The nitrogenous part of amino acids has the following functions

  • formation of urea for excretion of excess nitrogen
  • synthesis of other nitrogen-containing biomolecules

What is the exogenous and endogenous protein metabolism?

Exogenous protein metabolism refers to the digestion and absorption of dietary proteins

  • step 1 → digestion of proteins to amino acids
  • step 2 → absorption of amino acids into bloodstream Endogenous protein metabolism refers to the synthesis and breakdown of proteins within the body
  • step 1 → synthesis of proteins from amino acids
  • step 2 → breakdown of proteins to amino acids

What is the concept of enzyme, chemical reactions, catalyst and substrates?

Enzymes are biological catalysts that speed up chemical reactions

  • step 1 → binding of substrate to enzyme
  • step 2 → conversion of substrate to product
  • step 3 → release of product from enzyme

What are the major functions of enzymes?

Enzymes have the following functions

  • catalysis of chemical reactions
  • regulation of metabolic pathways
  • maintenance of tissue health

What is the importance of enzymes in the body?

Enzymes are important for

  • maintenance of homeostasis
  • regulation of metabolic pathways
  • synthesis and breakdown of biomolecules

What is the definition of vitamin?

Vitamins are organic compounds that are essential for maintenance of health

  • they are not synthesized by the body in sufficient amounts
  • they must be obtained from the diet

How are vitamins classified?

Vitamins are classified into

  • fat-soluble vitamins like vitamins A, D, E, and K
  • water-soluble vitamins like vitamins B and C

What are common vitamin deficiencies?

Common vitamin deficiencies include

  • vitamin A deficiency leading to night blindness
  • vitamin D deficiency leading to rickets
  • vitamin B12 deficiency leading to anemia

What is the use of different instruments in biochemistry experiments?

Different instruments are used for

  • measurement of pH and temperature
  • separation and identification of biomolecules
  • determination of concentration of biomolecules

How to perform the qualitative analysis of carbohydrate?

Qualitative analysis of carbohydrate involves

  • step 1 → preparation of sample
  • step 2 → addition of reagents
  • step 3 → observation of color change or precipitation

How to interpret the results of qualitative analysis of carbohydrate?

Results are interpreted based on

  • color change or precipitation
  • presence or absence of carbohydrate

How to perform the qualitative analysis of protein?

Qualitative analysis of protein involves

  • step 1 → preparation of sample
  • step 2 → addition of reagents
  • step 3 → observation of color change or precipitation

How to interpret the results of qualitative analysis of protein?

Results are interpreted based on

  • color change or precipitation
  • presence or absence of protein

How to perform the qualitative analysis of lipid?

Qualitative analysis of lipid involves

  • step 1 → preparation of sample
  • step 2 → addition of reagents
  • step 3 → observation of color change or precipitation

How to interpret the results of qualitative analysis of lipid?

Results are interpreted based on

  • color change or precipitation
  • presence or absence of lipid

How to perform the quantitative estimation of glucose?

Quantitative estimation of glucose involves

  • step 1 → preparation of sample
  • step 2 → addition of reagents
  • step 3 → measurement of absorbance or concentration

How to interpret the results of quantitative estimation of glucose?

Results are interpreted based on

  • concentration of glucose
  • comparison with standard values

How to perform the quantitative estimation of total proteins?

Quantitative estimation of total proteins involves

  • step 1 → preparation of sample
  • step 2 → addition of reagents
  • step 3 → measurement of absorbance or concentration

How to interpret the results of quantitative estimation of total proteins?

Results are interpreted based on

  • concentration of total proteins
  • comparison with standard values

How to observe the quantitative estimation of uric acid?

Quantitative estimation of uric acid involves

  • step 1 → preparation of sample
  • step 2 → addition of reagents
  • step 3 → measurement of absorbance or concentration

How to interpret the results of quantitative estimation of uric acid?

Results are interpreted based on

  • concentration of uric acid
  • comparison with standard values

How to observe the laboratory testing for lipid profile?

Laboratory testing for lipid profile involves

  • step 1 → preparation of sample
  • step 2 → measurement of lipid components
  • step 3 → interpretation of results

How to interpret the results of lipid profile testing done in a laboratory?

Results are interpreted based on

  • concentration of lipid components
  • comparison with standard values
  • presence or absence of lipid disorders